Can a DNA ladder help determine?

DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories.

Why did my DNA ladder smear?

Smearing of DNA ladder occurs due to degradation of DNA into smaller fragments. It can result due to improper storage or due to used running buffer. As far as DNA bands in other wells are concerned, their curved nature results from bad gel casting.

What is the function of a DNA size ladder?

A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel …

How do you dilute 1 kb DNA ladder?

Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).

How much dye do you put on a DNA ladder?

The recommended loading volume of the 1 kb DNA ladder is 6 μL per lane. DNA Loading Dye is a ready-to-use marker dye containing orange G (0.4%), bromophenol blue (0.03%), and xylene cyanol FF (0.03%), in 15% Ficoll® 400, 10 mM Tris-HCl (pH 7.5), and 50 mM EDTA (pH 8.0).

How big is the generuler 1 KB plus DNA ladder?

Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels.

When did Thermo Fisher upgrade their DNA ladder?

This product was upgraded on September 15, 2017. Learn more at .

How many DNA fragments are in a DNA ladder?

The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation.

How many DNA fragments are in Invitrogen 1 KB plus?

Invitrogen E-Gel 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. E-Gel 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.