How do you reduce cost in downstream processing?
One way to achieve this is through adoption of higher-efficiency chromatography sorbents and membranes that reduce consumption, disposal, and recycling of costly salts, as well as the use of cleaning agents and water, while effectively removing host cell proteins, DNA, viruses, and other impurities.
What are the basic strategies of downstream processing?
The process consists of only three main steps: 1) simultaneous extraction and transesterification of the algal biomass; 2) argentated silica gel column chromatography of the crude extract; and 3) removal of pigments by a second column chromatographic step.
Why is downstream processing expensive?
The fermentation processes that are used by biopharmaceutical manufacturers have shown to lead to increasing quantities of therapeutic proteins. However, this increase in turn leads to capacity bottlenecks in the subsequent purification process (known as downstream processing) and is associated with high costs.
Which are the downstream processes?
Downstream processing refers to the recovery and the purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal or plant tissue or fermentation broth, including the recycling of salvageable components and the proper treatment and disposal of waste.
What is extraction in downstream processing?
Extraction. It involves the recovery of a compound or a group of compounds from a mixture or from cells into a solvent phase. It usually achieved both separation of particles as well as concentration of the product.
Why downstream processing is important?
4.2. Downstream processing involves multi-step procedures for the recovery and purification of products of interest . From a commercial point of view, the most important objective in downstream processing is to maximize product recovery and, at the same time, minimize the cost of production .
How do I improve downstream processing?
High flow rates can improve turnaround time in downstream processing by decreasing washing, cleaning, and re-equilibration times. In most cases, sample loading and elution times can also be reduced, though this reduction depends on the properties of the target molecule and impurities.
What is ultrafiltration in downstream processing?
Protein ultrafiltration is a pressure-driven membrane process used for the concentration and/or purification of protein solutions. Ultrafiltration is currently used throughout downstream processing for protein concentration, buffer exchange and desalting, protein purification, virus clearance, and clarification.
Which chromatography is used in bioprocessing?
Multi-column chromatography techniques such as simulated moving bed (SMB) allow for semi-continuous operation of a chromatography process. These approaches are widely used in other process industries and are beginning to be considered for biopharmaceutical processes.
Which operations can be used for removal of insolubles?
Typical harvesting operations for separation of insolubles (whole cells) are centrifugation (mainly for yeast but also for mammalian cells and bacteria), rotary vacuum filtration (mainly fungii) and microporous filtration (bacteria, yeast, mammalian cells and also fungii).
What are the challenges of the downstream manufacturing process?
The downstream manufacturing process takes the output from upstream operations and transforms it through multiple steps into a viable drug product ready for administration to patients. The major challenge in downstream processing is to maximize yield while meeting both product and impurity specifications.
What are the results of a cost cutting strategy?
The results were profound: New and clearly-defined roles improved support operations both in the branches and the back office. The customer experience improved. More than 30 percent of processing capacity was recovered. That’s a cost cutting strategy that really pays off.
How is filtration used in the downstream process?
Downstream Process Unit Operations Process filtration Filtration is used at several stages in the downstream processing of the bioreactor harvest, as well as for the preparation of purified water and other processing fluids (buffers, sanitizing agents, etc.). Several filtration steps are integral to the Capture, Intermediate purification, and
What are the goals of the downstream process?
The downstream process can be divided into three stages, each with the primary goals of the corresponding stage as follows: Captureor Recovery: This involves the rapid separation of the product of interest from the cells of the bioreactor.