How will you isolate hydrocarbon degrading bacteria?
The hydrocarbon degraders which are stored on glycerol stock were subjected to its efficiency of crude oil degradation. The isolates were single streaked on BHA plates overlaid with 100 µl of crude oil and on plain NA plates and were incubated at 25°C for 14 days and 37°C for 24 – 48 hrs respectively.
What are hydrocarbon degrading microbes?
Petroleum hydrocarbon-degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Bacteria displaying such capabilities are often exploited for the bioremediation of petroleum oil-contaminated environments.
What is the benefit of a microorganism that can degrade hydrocarbons and are the ones available currently effective?
Since microorganisms possess the ability to decompose hydrocarbons as an energy source; their application in bioremediation processes is a natural consequence.
What is hydrocarbon bioremediation?
The ability of microorganisms – bacteria, archaea, fungi, or algae – to break down hydrocarbons is the basis for natural and enhanced bioremediation. To promote biodegradation, amendments such as nitrogen and phosphorous fertilizer are often added to stimulate microbial growth and metabolism.
Why do microbes degrade contaminants?
Microbes degrade contaminants because in the process they gain energy that allows them to grow and reproduce. Microbes get energy from the contaminants by breaking chemical bonds and transferring electrons from the contaminants to an electron acceptor, such as oxygen.
How can we prevent hydrocarbon pollution?
The remediation of hydrocarbon polluted wastewater can be achieved by three methods, which are phytoremediation, bioremediation and chemical remediation. Phytoremediation entails the use of plants to reduce the volume, mobility and toxicity of contaminants in soil and water.
Why the isolation of pure compounds is extremely difficult from petroleum?
The isolation of pure compounds from petroleum is an exceedingly difficult task, and the overwhelming complexity of the hydrocarbon constituents of the higher-molecular-weight fractions and the presence of compounds of sulfur, oxygen, and nitrogen are the main causes for the difficulties encountered.
What is the role of microbes in reducing environmental degradation caused by chemicals?
To reduce the environmental degradation caused by chemicals, microbes can be used both as fertiliseres and pesticides and can be called biofertilisers and biopesticides, respectively Microbes are used as biofertilisers to enrich the soil nutrients, e.g., Rhizobium Azotobacter, Azospirillum, etc, which can fix …
Which organism is useful in degrading organic pollutants?
Fungi are an important part of degrading microbiota because, like bacteria, they metabolize dissolved organic matter; they are principal organisms responsible for the decomposition of carbon in the biosphere.
How can we reduce hydrocarbons?
What possible effect do hydrocarbons may bring to health and environment if it will be used without proper regulation?
As major components of oil, natural gas and pesticides, these substances contribute to the greenhouse effect and climate change, deplete the ozone, reduce photosynthetic ability of plants, and increase occurrences of cancer and respiratory disorders in humans.