What are sources of contamination in plant tissue culture?
Contamination in tissue culture can originate from two sources, either through carry over of microorganisms on the surface or in the tissues of explants, or through faulty procedures in the laboratory. Plant surfaces are habitats for microorganisms (Campbell, 1985).
How do you identify contamination in plant tissue culture?
Fungal, bacterial, and yeast contaminations are among the easiest to identify. But while they are easy to identify, they can be vicious unwelcome guests. If you are using agar or another medium with added phenol red, then be on alert if your medium changes color as this indicates microbial contamination.
What are the four types of plant tissue culture?
Types of Plant tissue culture
- Seed Culture.
- Embryo Culture.
- Callus Culture.
- Organ Culture.
- Protoplast Culture.
- Anther Culture.
What are the major difficulties in plant tissue culture method?
There are some major problems in practical application of plant tissue culture such as: browning of cultured tissues, vitrification of tissues, bacterial and fungal contaminations, deterioration of embryonic callus and its inability to form embryos, callus formation on bases of rooting plantlets.
What is explant 12th?
Explant is any part of plant like cell, tissue or organ grown in a test tube containing artificial nutrient medium under sterile condition. Totipotencyis the capacity of a plant cell to multiply and regenerate into a new plant.
What are the different types of tissue culture?
Following are the different types of tissue culture techniques:
- Seed Culture. In this culture, the explants are obtained from an in-vitro derived plant and introduced into a laboratory where they proliferate.
- Embryo Culture.
- Callus Culture.
- Organ Culture.
- Protoplast Culture.
- Other Types.
- Initiation Phase.
- Multiplication Phase.
How can you prevent contamination in plant tissue culture?
How To Prevent Cell Culture Contamination
- Wear gloves, lab-coats and use hoods.
- Use your hood correctly.
- Clean your incubator and water bath regularly.
- Spray EVERYTHING with ethanol or IMS.
- Minimize exposure of cells to non-sterile environments.
What are disadvantages of tissue culture?
Disadvantages of Tissue Culture
- Tissue Culture can require more labor and cost more money.
- There is a chance that the propagated plants will be less resilient to diseases due to the type of environment they are grown in.
What are the kinds of contamination are faced during cell culture?
This section provides an overview of major types of biological contamination: Bacterial Contamination. Mold & Virus Contamination. Mycoplasma Contamination.
