What are the principles involved in the biuret and ninhydrin test for proteins?

Biuret Test: The Biuret Test is a general test for proteins. When a protein reacts with copper(II) sulfate (blue), the positive test is the formation of a violet colored complex. When such an -NH2 group reacts with ninhydrin, a purple-blue complex is formed.

What happens when ninhydrin react with a protein?

Ninhydrin reacts with the α-amino group of primary amino acids producing ‘Ruhemann’s purple’. The chromophore formed is the same for all primary amino acids. The intensity of the colour formed depends on the number and chemical nature of the amino groups being analysed. The optimum pH for the overall reaction is 5.5.

What is the reaction involved in ninhydrin test?

This test is due to a reaction between a amino group of free amino acid and ninhydrin. The NH3 formed from a amino group reacts with another molecule of ninhydrin and is reduced product ( hydrindatin) to give a blue substance diketohydrin ( Ruhemanns complex).

What is the purpose of ninhydrin test?

The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or α-amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte.

What is the aim of ninhydrin test?

Ninhydrin test is used to detect the presence of amino acids in unknown samples. This test is also used in solid-phase peptide synthesis to monitor the protection for amino acid analysis of proteins. As the ninhydrin test is quite sensitive, it is commonly used to detect fingerprints.

Which is a better test for proteins biuret or ninhydrin?

The Ninhydrin test displayed a minimum level of detection observed by 75% of volunteers (MLD(75)) of 9.25 microg [95% confidence interval (95% CI) 8.6-10.0 microg]. The Biuret test provided better sensitivity, with a MLD(75) of 6.7 microg (95% CI 5.4-8.2 microg).

What is the principle of ninhydrin?

Ninhydrin Test Principle The amino group belonging to a free amino acid undergoes a chemical reaction with ninhydrin, which behaves as an oxidizing agent. When the analyte contains Imino-acids like proline, a yellow coloured complex is formed. When asparagine is used, the colour of the resulting complex is brown.

Is ninhydrin test positive for all proteins?

Ninhydrin Test Almost all amino acids contain a free amino group (except proline and hydroxyproline). Some proteins also give a positive test with ninhydrin.