What is a DC protein assay?
Description. The DC (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization. Using standard procedure, the assay is used with samples having protein concentrations between 0.2 and 1.5 mg/ml and requires just 100 µl of sample.
How does DC protein assay work?
This assay is based upon a simple reaction whereby the protein within a solution reacts with a Folin reagent and an alkaline copper tartate solution. The outcome of this reaction is the formation of a blue product (the more protein, the darker the blue).
What is the Folin Lowry method?
A sensitive method of protein assay in which protein reacts both with an alkaline copper tartrate solution and with the Folin‐Ciocalteu reagent to give a blue colour, the absorbance of which is measured at 750 nm.
What is the most accurate protein assay?
BCA assay
Compared to other methods, the BCA assay is one of the most sensitive (it can detect proteins at concentrations as low as 5 ug/mL). It has less variability than others (i.e., Bradford assay), and it can be used to measure a wide range of protein concentration.
How do you make Lowry reagent?
The Lowry Reagent Solution is prepared by adding 40 ml of water to a bottle of Lowry Reagent, Powder (Product Code L 3540). Mix well by inverting to completely dissolve the contents. Do not shake so as to minimize foaming.
Which method is best for protein estimation?
The simplest and most direct assay method for protein concentration determination in solution is to measure the absorbance at 280 nm (UV range). Amino acids containing aromatic side chains (i.e., tyrosine, tryptophan and phenylalanine) exhibit strong UV-light absorption.
Is Lowry better than Bradford?
The Bradford assay is is the fastest and easiest to perform among the protein assays and uses about the same amount of protein as the Lowry assay. The difference between the two forms of the dye is greatest at 595 nm, so that is the optimal wavelength to measure the blue colour from the Coomassie dye-protein complex.
Why we use BSA as standard for protein estimation?
BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
What is the Bio-Rad DC protein assay used for?
The Bio-Rad DC Protein Assay is a colorimetric assay for protein concen- tration following detergent solubilization.
How long does a DC protein assay last?
The DC protein assay requires only a single 15 minute incubation, and absorbance is stable for at least 2 hours. Using standard procedure, the assay is used with samples having protein concentrations between 0.2 and 1.5 mg/ml and requires just 100 µl of sample.
How is the DC ( detergent compatible ) protein assay used?
The DC (detergent compatible) protein assay uses a colorimetric method to determine protein concentration following detergent solubilization. The reaction is similar to the well-documented Lowry assay.
Is the RC DC protein assay based on Lowry protocol?
The RC DC protein assay has all the unique features of the original DC protein assay, but covers a broader range of reagents. This assay is based on the Lowry protocol, and its expanded capabilities offer:
