What is Agilent microarray?
As I will explain, Agilent microarrays are made using DNA, so we call them DNA microarrays, or gene arrays. The measurement and study of this DNA is called genomics. The piece parts of the cell are a class of molecules called proteins. Each cell contains thousands of tiny factories that make proteins.
What is a CGH microarray test?
Array CGH (also known as microarray, or chromosome microarray (CMA)) is an ultra-high resolution way of objectively and quantitatively detecting whether a patient’s DNA has losses (deletions) or gains (duplications, triplications etc) which are pathogenic and therefore explain their clinical problems.
What is the function of the restriction enzyme in the CGH analysis?
STEP 1: RESTRICTION DIGESTION: The purpose of this step is to fragment the high molecular weight genomic DNA (gDNA) into smaller pieces. Restriction digestion is required for SNP analysis, while other protocols may use other methods, such as sonication, to fragment the DNA.
What is microarray platform?
Microarrays have allowed gene expression profiling to progress from the gene level to the genome level, and oligonucleotide microarrays have become the platform of choice for large-scale, targeted gene expression studies. This microarray has greater coverage of mycobacterial genes than any other array reported to date.
What is CGH technique?
Comparative genomic hybridisation (CGH) is a technique that permits the detection of chromosomal copy number changes without the need for cell culturing. It provides a global overview of chromosomal gains and losses throughout the whole genome of a tumour.
What are CGH arrays used for?
Array CGH applications are mainly directed at detecting genomic abnormalities in cancer. However, array CGH is also suitable for the analysis of DNA copy number aberrations that cause human genetic disorders. That is, array CGH is employed to uncover deletions, amplifications, breakpoints and ploidy abnormalities.
What is the difference between CGH and array CGH?
Array CGH is automated, allows greater resolution (down to 100 kb) than traditional CGH as the probes are far smaller than metaphase preparations, requires smaller amounts of DNA, can be targeted to specific chromosomal regions if required and is ordered and therefore faster to analyse, making it far more adaptable to …
How does CGH work?
How does array CGH work? The patient and reference DNA are labelled with different coloured fluorescent dyes and applied to an array slide on to which is spotted DNA representing the whole genome. The patient and reference DNA binds to the DNA on the slide.
What can a microarray detect?
A microarray is the recommended first line genetic test for developmental delay (DD), intellectual disability (ID) and autism spectrum disorders (ASD)*. CMA however, does not identify fragile X syndrome (FXS), a common cause of intellectual disability.
How do you perform a microarray?
To perform a microarray analysis, mRNA molecules are typically collected from both an experimental sample and a reference sample. For example, the reference sample could be collected from a healthy individual, and the experimental sample could be collected from an individual with a disease like cancer.
Under what applications are microarrays useful?
It helps especially in the identification of single-nucleotide polymorphisms (SNPs) and mutations, classification of tumors, identification of target genes of tumor suppressors, identification of cancer biomarkers, identification of genes associated with chemoresistance, and drug discovery.
When to use Agilent CGH for DNA analysis?
Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis Bravo Automated Liquid Handling Platform with Enzymatic and ULS Labeling Protocol Version 2.3, August 2015 For Research Use Only. Not for use in diagnostic procedures. Array-Based CGH for Genomic DNA Analysis – Bravo Automated Liquid Handling Platform Notices
How many probes are in a Cgh microarray?
Agilent’s CGH platform provides unmatched flexibility on content and throughput, thanks to eight different microarray formats with variable density and arrays per slide. Agilent provides a database of more than 28 million validated probes, and easy tools to customize array content based on needs.
How many probes are in a Agilent microarray?
Agilent provides a database of more than 28 million validated probes, and easy tools to customize array content based on needs. Custom or catalog, all Agilent’s CGH & CGH+SNP arrays benefit from proprietary SurePrint technology with long, high quality oligos. CGH & CGH+SNP Microarrays
What can a Cgh + SNP microarray be used for?
Learn about our extensive range of CGH + SNP microarrays dedicated to cytogenetic workflows. We provide whole genome CMA at variable resolutions, down to exon level, CGH+SNP arrays to analyze CN and LOH in a single experiment and application specific designs, including pre- post-natal, PGS, CNV and Cancer specific microarrays.