What is pGL3?
Description. The pGL3 Luciferase Reporter Vectors(a) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors.
What is pGL3 basic vector?
Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay.
What is a luciferase vector?
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may be cis- or trans-acting factors. The assay of this genetic reporter is rapid, sensitive and quantitative.
What is pcdna3?
This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
What is the purpose of the luciferase gene in the reporter vector?
A commonly used reporter gene is the luciferase gene from the firefly Photinus pyralis. This gene encodes a 61-kDa enzyme that oxidizes D-luciferin in the presence of ATP, oxygen, and Mg(++), yielding a fluorescent product that can be quantified by measuring the released light.
Where does luciferase come from?
Luciferase is a light-producing enzyme naturally found in insect fireflies and in luminous marine and terrestrial microorganisms.
What is pCMV plasmid?
The pCMV-GLuc 2 Control Plasmid is a mammalian expression plasmid that constitutively expresses the secreted Gaussia luciferase (GLuc) under the control of the CMV promoter. View sequence details. Strong constitutive expression of GLuc. GLuc is codon-optimized for mammalian expression.