What is the difference between MCF-7 and MDA-MB-231 cell lines?
MDA-MB-231 is triple negative breast cancer cell lines and they are about 15% to 20% of all breast cancer cases and hard to treat them. Need more research for discovery of new drugs or compounds. MCF-7 is estrogen receptor positive breast cancer cell lines and effective treatments drugs are available.
What is the doubling time for MDA-MB-231?
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay.
What are bt474 cells?
Breast Cancer Model BT-474 This expression profile makes them a prevalent model to develop new breast cancer therapeutic agents. BT-474 cells grow in response to estradiol but shows resistance to ER antagonist tamoxifen. Estradiol supplement is required to establish xenograft model of athymic nude mice.
What is MCF-7 cell line?
MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors . It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI .
Does MCF-7 have HER2?
Two particularly important members of this large family are the epidermal growth factor receptor (EGFR), which is activated by the epidermal growth factor (EGF), and the human epidermal growth factor receptor-2 (HER2), both present in MCF-7 cells (7).
Are MCF-7 cells metastatic?
All Answers (7) No, MCF-7 is considered metastatic. The non-tumorous and non-metastatic line is MCF-10A or MCF-12A.
Is MCF 7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What is DU145?
DU145 (DU-145) is a human prostate cancer cell line. DU145, PC3, and LNCaP are considered to be the standard prostate cancer cell lines used in therapeutic research.
Who makes trastuzumab?
Genentech: Herceptin® (trastuzumab) – Information for Patients.
Is MCF7 triple negative?
Are MCF7 cells metastatic?
What is the MDA-MB-231 breast cancer cell line?
MDA-MB-231 (ECACC catalogue no. 92020424) Cell line history. The MDA-MB-231 cell line is aepithelial,n human breast cancer cell line that was established from a pleural effusion year-old caucasian femaleof a 51 with a metastatic mammary – adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.
How are MDA-MB-231 ME cells grown?
MDA-MB-231 cells are grown at 37◦C in Leibovitz’s L-15 medium supplemented with 2mM glutamine and 15% foetal bovine serum (FBS). dium supports the growth of This me cells in environments without CO 2.
Which is the best cell line for breast cancer?
The cell line (MDA-MB-231) with the highest PIWIL4 expression was chosen as the cell line model for breast cancer. Reduction of PIWIL4 expression was observed to significantly decrease EMT and cell migration, increase apoptosis, and reduce cell proliferation in this cell line.
Is the m.indica L extract cytotoxic to breast cells?
Al-Shwyeh et al. (2014) evaluated the cytotoxicity of ethanolic kernel extract of M. indica L on MCF-7 and MDA-MB- 231 cell lines and concluded that the extract is significantly cytotoxic to these cell lines in a dose-dependent manner, and considerably less so toward normal breast cells MCF-10A.