What is the normal reticulocyte production index?
between 0.5% and 2.5%
The reticulocyte index (RI) should be between 0.5% and 2.5% for a healthy individual. RI < 2% with anemia indicates maturation disorder, meaning loss of red blood cells, but also decreased production of reticulocytes (i.e., an inadequate response to correct the anemia) and therefore red blood cells.
How do you calculate reticulocyte production index?
RPI method 1
- Reticulocyte Index = Reticulocyte count x Hematocrit / Normal Hematocrit.
- RPI = Reticulocyte index/ Maturation correction.
- RPI = Reticulocyte count x Hemoglobin / Normal Hemoglobin x 0.5.
What is a high reticulocyte count in dogs?
The presence of increased numbers of reticulocytes indicates that the anemia is responsive. This means the body has identified anemia (responding) and is attempting to correct the deficit by releasing immature red blood cells.
How do you correct a reticulocyte count?
Because the reticulocyte count is expressed as a percentage of total RBCs, it must be corrected according to the extent of anemia with the following formula: reticulocyte % × (patient Hct/normal Hct) = corrected reticulocyte count.
How do you fix reticulocyte count?
In patients with severe anemia, reticulocytes leave the marrow early and last longer in the peripheral blood. A simple way to correct for this is to divide the reticulocyte count in half if the HGB is less than 10 (and the HCT less than 30).
How do you do a reticulocyte count?
When you get this test, a lab tech will take a sample of blood from one of your veins. In earlier years, doctors would put a drop of blood on a microscope slide and count the number of reticulocytes themselves. Today, machines calculate the results of nearly all reticulocyte count tests.
What causes dog reticulocytosis?
Pathologic causes of reticulocytosis usually result from an increase in bone marrow production of red blood cells secondary to external or internal blood loss and hemolytic disease.
What does reticulocyte count indicate?
These red blood cells move oxygen from your lungs to every cell in your body. A reticulocyte count (retic count) measures the number of reticulocytes in the blood. If the count is too high or too low, it can mean a serious health problem, including anemia and disorders of the bone marrow, liver, and kidneys.
Which stain is used for reticulocyte staining?
new methylene blue
A supravital stain, such as new methylene blue N or brilliant cresyl blue, is used to stain reticulocytes (indicated by the arrows in Image B) for an actual count.
How is the reticulocyte count in a dog determined?
The percentage reticulocyte count requires, at least, conversion to absolute values. The reticulocyte count in the dog requires several conversions to account for the “normal” bone marrow response to erythropoietin, and the length of time of bone marrow reticulocyte maturation which is correlated with the degree of anemia.
What is the red cell production index for dogs?
Reference ranges for CRP are 1% in the dog and 0.4% in the cat. Red cell production index (RPI) Calculation of the RPI takes into account the degree of anemia and the rate of erythrocyte maturation in the circulation.
What is normal reticulocyte production index for HCT?
Maturation = 1.5 for Hct 30-39.9%. Maturation = 2.0 for Hct 20-29.9%. Maturation = 2.5 for Hct <20%. An RPI >3 shows a normal marrow response to anemia. An RPI <2 is an inadequate response to anemia. With a normal H&H, an RPI of 1 is normal.
How does the RPI work in a dog?
Calculation of the RPI takes into account the degree of anemia and the rate of erythrocyte maturation in the circulation. This assumes that the predicted lifespan of reticulocytes is similar in human beings and dogs. Although this may not be true, the RPI does seem to work in the dog only.