What is the restriction site of Eco r1?
Additional Information: The EcoRI is a restriction enzyme that creates four nucleotide sticky ends with the end of 5′. The enzyme cuts at the recognition site of G/AATTC which has a complementary sequence of CTTAA/G.
What is the cut site for EcoRI?
In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5′ end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic, complementary sequence of CTTAA↓G.
How many restriction sites are there for EcoRI?
Note, after a reaction with the EcoRI enzyme, that the DNA of species A is cleaved into three fragments, corresponding to two EcoRI restriction sites, whereas that of species B is cleaved into four fragments, corresponding to three EcoRI restriction sites.
How do I find restriction sites?
The option Find Restriction Sites… from the “Tools”→“Cloning” menu or the context menu allows you to find and annotate restriction sites on a nucleotide sequence.
What is the recognition site of restriction enzyme?
Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes.
How many restriction sites do plasmids have?
A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites – a standard feature of engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid.
Are restriction sites palindromic?
Restriction-modification systems are used as a defensive mechanism against inappropriate invasion of foreign DNA. The recognition sequences for the common type II restriction enzymes and their corresponding methylases are usually palindromes.
How many recognition sites are there in pBR322?
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !- lactamase (AmpR) gene and eight unique sites within the TetR gene.
What is a palindrome and how does it apply to restriction enzymes?
A palindromic sequence is the same backwards and forwards on both sides (see image below). This means that the enzyme recognizes the sequence no matter from which side the enzyme approaches the DNA. A palindromic sequence also increases the chance that both strands of DNA are cut.