How does replication stop?
Termination requires that the progress of the DNA replication fork must stop or be blocked. Termination at a specific locus, when it occurs, involves the interaction between two components: (1) a termination site sequence in the DNA, and (2) a protein which binds to this sequence to physically stop DNA replication.
Why do replication forks pause?
Pausing of replication forks at accidental barriers, such as Tet repressor/operator arrays in E. coli, might simply result from the physical clash between the replisome and the protein–DNA complex at the RFB.
What are the three phases of replication?
Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment.
What is replication slippage describe with example?
Slipped strand mispairing (SSM), (also known as replication slippage), is a mutation process which occurs during DNA replication. When DNA polymerase encounters a direct repeat, it can undergo a replication slippage. Strand slippage may also occur during the DNA synthesis step of DNA repair processes.
What is stalled replication fork?
In order for the replication fork to stall, the cell must possess a certain number of stalled forks and arrest length. The replication fork is specifically paused due to the stalling of helicase and polymerase activity, which are linked together.
Why is replication called Semiconservative?
DNA replication is semi-conservative because each helix that is created contains one strand from the helix from which it was copied. The replication of one helix results in two daughter helices each of which contains one of the original parental helical strands.
What is meant by replisome?
Definition. The replisome is a large protein complex that carries out DNA replication, starting at the replication origin. It contains several enzymatic activities, such as helicase, primase and DNA polymerase and creates a replication fork to duplicate both the leading and lagging strand.
What happens at replication fork?
The replication fork * is a region where a cell’s DNA * double helix has been unwound and separated to create an area where DNA polymerases and the other enzymes involved can use each strand as a template to synthesize a new double helix.
What are the 3 main stages of Semiconservative replication?
There are three main steps to DNA replication: initiation, elongation, and termination.
What is DNA replication slippage?
Replication slippage or slipped-strand mispairing involves the misalignment of DNA strands during the replication of repeated DNA sequences, and can lead to genetic rearrangements such as microsatellite instability.